The UltraScence Atto Western Substrate, as a luminol-based enhanced chemiluminescent substrate, is the most sensitive and brightest ECL Western Substrate among our UltraScence product lines for low-femtogram to high-attogram detection of antigen with excellent sensitivity and long signal duration. UltraScence Atto Western Substrate is compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugated secondary antibodies. Further, its long chemiluminescent signal duration makes both digital and film-based imaging possible without any loss of the signal.

Product Name: UltraScence Atto Western Substrate
Product Item No.: CCH385-B100ML
Size: 100ml
Substrate Type: HRP (Horseradish Peroxidase) Substrate
Contents/Storage: Stable for up to 24 months at 4°C
US Patent No.: US10,711,185

- No optimization required.
Switching to the UltraScence Atto Western Substrate from other brands, such as Thermo Scientific™ SuperSignal™ West Atto, does not require optimization or protocol changes.

- High degree of sensitivity and enhanced chemiluminescence duration.
UltraScence Atto Western Substrate enables an accurate low-femtogram to high-attogram detection of protein on the same immunoblot after a single exposure.

- Optimized for use with PVDF and nitrocellulose membranes.
- Compatible with Western Blotting Markers.
- Optimized for film- and CCD-based imaging.
- Patent pending product.

**US Patent No.: US10,711,185

Figure 1. The PVDF membranes were spotted with serial dilution HRP and probed with Goat Anti-rabbit IgG/HRP secondary antibody (1:10,000). Identical blots were incubated with the Western substrate. The blots were simultaneously exposed for 5 seconds, 10 seconds, 30 seconds, 60 seconds, and 180 seconds using Chemlux SPX-600 Series digital imaging system.

Figure 2. Membranes were probed with GFP tag Rabbit PolyAb diluted at 1:10,000 of and then with Goat Anti-rabbit IgG/HRP secondary antibody (1:10,000) after serial dilution EGFP (Enhanced Green Fluorescent Protein) were prepared and applied in electrophoresis and protein transfer. Identical blots were incubated with the Western substrate. The blots were simultaneously exposed for 5 seconds, 10 seconds, 30 seconds, 60 seconds, and 180 seconds using ChemluxSPX-600 Series digital imaging system.

* SuperSignal West Atto is a registered trademark of Thermo Fisher Scientific. The trademark holder is not affiliated with Bio-HeliX Co., Ltd. and does not recognize this product.

Q: From Bio-Helix’s UltraScence ECL substrates portfolio, which level of ECL fits best my Western blot applications?
A: UltraScence ECL substrates series is compatible with the use from low picogram to low-femtogram level detections. Please kindly refer to the ECL selection guide of UltraScence Western substrate as the below table:

(a) CCH321-B100ML, UltraScence Pico Plus Western Substrate
(b) CCH345-B100ML, UltraScence Pico Ultra Western Substrate
(c) CCH365-B100ML, UltraScence Femto ECL Western Substrate

(d) CCH375-B100ML, UltraScence Femto Plus ECL Western Substrate

(e) CCH385-B100ML, UltraScence Atto ECL Western Substrate