BIO-HELIX - DN001-0250

100 mM dNTP Set

Size: 4 x 250 μl



Note: Deoxynucleotide Solution Set / PCR Grade / dATP, dCTP, dGTP, dTTP
Categories: PCR Reagent

100 mM dNTP (2′-deoxynucleoside 5′-triphosphate) Set consists of all four deoxynucleotides (dATP, dCTP, dGTP, dTTP), each at a concentration of 100 mM. The deoxynucleotides are suitable for use in polymerase chain reaction (PCR), sequencing, fill-in, nick translation, cDNA synthesis, and TdT tailing reactions. The product is supplied as ready-to-use solutions.

The 100 mM dNTP Set includes 100 mM each of dATP, dCTP, dGTP, and dTTP.
Each vial contains 250 μl of 25 μmol dNTP in purified water (pH 7.5).

20 μl Final Reaction Volume

Final dNTP Conc dNTP Volume
0.2 mM 0.04 μl
0.5 mM 0.1 μl
1.0 mM 0.2 μl
1.5 mM 0.3 μl

 

25 μl Final Reaction Volume

Final dNTP Conc dNTP Volume
0.2 mM 0.05 μl
0.5 mM 0.125 μl
1.0 mM 0.25 μl
1.5 mM 0.375 μl

 

50 μl Final Reaction Volume

Final dNTP Conc dNTP Volume
0.2 mM 0.1 μl
0.5 mM 0.25 μl
1.0 mM 0.5 μl
1.5 mM 0.75 μl