The Blu12 Prestained Protein Ladder is a combination of 12 pre-stained proteins with molecular weights from 11 to 245 kDa. The 12 recombinant proteins are covalently coupled with blue chromophore, while 1 green band at 25 kDa and a red band at 75 kDa serve as reference bands. The Blu12 Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.

Guide for Molecular Weight Estimation (kDa)
Refer to the Blu12 Prestained Protein Ladder patterns in various electrophoresis conditions:

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.

Storage Buffer : 
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol.

Storage : 
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
Stable for up to 24 months at -20°C.

Lane 1: PMB11-0500 (Bio-Helix) 5ul
Lane 2: PMB13-0500 (Bio-Helix) 5ul
Lane 3: PMB12-0500 (Bio-Helix) 5ul
Lane 4: PMB10-0500 (Bio-Helix) 5ul
Lane 5: PMB01-0500 (Bio-Helix) 5ul
Lane 6: PMB11-0500 (Bio-Helix) 5ul
Lane 7: PMB01-0500 (Bio-Helix) 5ul
Lane 8: PMU12-0500 (Bio-Helix) 5ul
Lane 9: thermo Mark12 5ul
Lane 10: Bio-Rad 161-0317 5ul

*NuPAGE Bis-Tris 4-12% was used to run SDS-PAGE in MOPS and MES buffer. 5ul of protein ladder samples was loaded into each lane.

• Vertical Electrophoresis system
• Power supplies
• Vortex or equivalent
• Microcentrifuge