Prime Juice Keeps Your Lab Safe

• Safe
  Absence of mutagenicity
• Low Environmental Impact
  Compliance with the Clean Water Act standards. No water pollution concern. Sensitivity – High degree of sensitivity as Ethidium Bromide.
• Convenience
  Ready to Use; Same application procedures as the 6X Loading Dye.
• Speed
  No de-staining requirement, low background value, an image displayed after coupling with the nucleic acid.
• Compatibility
  Use the Blue Light or UV to detect the signal; Broad compatibility range.
• Economic
  Non-hazardous product; No expenses required for the waste management.
• Better Sensitivity
  Increase fluorescence intensity.

Prime Juice is a non-mutagenic fluorescent reagent producing an instant visualization of DNA bands upon illuminating agarose gels on the Blue Light Transilluminator (~470 nm) or UV Transilluminator (~302 nm). Prime Juice, as supplied in the 6X DNA Loading Buffer, is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains three tracking dyes (Bromophenol Blue, Xylene Cyanol FF, and Orange G) for visually tracking the DNA migration during the electrophoresis process and for detecting the double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), and RNA at as low as 0.14 ng DNA, thus rendering it the most sensitive stain available on the market.

The recommended DNA sample mass is at least 50 ng or more, thus not causing any obvious shift in the migration pattern. Regardless of the loaded DNA or RNA sample amount for staining, it achieves cogently consistent migration pattern without the “smiley signature” commonly identified with other competing stains on the market. It is the ideal non-hazardous alternative to Ethidium Bromide for protecting the environment and meeting with the local biosafety regulations/ESG practices/UN SDG Goals.

Figure 1. Each lane was loaded with 5ul of 100 bp DNA Ladder H3 (DM003-R500) and 1ul of Prime juice (LD011-1000) and Novel Juice (LD001-1000) in serial dilution. At low sample concentrations, Prime juice shows better sensitivity, as elucidated by the comparison data.

Figure 2. Compatibility test
DNA Ladder (DM003-R500, Bio-Helix) was run in 2% agarose gel (MB755-0100, Bio-Helix) with TAE buffer loaded with the premix of Prime Juice (LD011-1000, Bio-Helix) from Lane A-D, as well as Novel Juice (LD001-1000, Bio-Helix) from Lane E-H. The gel images were taken by different devices to show the appearances of both dyes.