AmpFAST 2X PCR SuperMix was premixed with an optimized concentration of AmpFAST Taq DNA Polymerase, dNTPs, Mg²⁺, and reaction buffer that could be used in extremely fast PCR experiments as well as massive gene detection requirements, which also performs better amplification functions for GC-rich and complicated secondary structures. For shorter sequence or plasmid samples, it is suggested to use 5 sec/kb elongation speed or less circuits to shorten your PCR reacting time. While for longer sequences (≥3 kb) or complicated samples that usually have lesser PCR products, it is suggested to use 15-30 sec/kb elongation speed or more circuits. Meanwhile, an “A” base at the 3’-end of the PCR products amplified by this enzyme can be directly used in TA cloning.

Figure 1. Amplification Results Using MB202-P040 on Templates from Different Sources
(A) E. coli (3 kb) (B) Soybean Genomic DNA (1.5 kb) (C) Mouse Genomic DNA (3 kb) (D) λDNA (1.2 kb) (E) Mouse Genomic DNA (1 kb, GC-rich)
Groups A-C were amplified at a speed of 15 sec/kb, while groups D-E were amplified at 5 sec/kb.