For analyzing the small amounts of proteins (down to 10 pmoles), peptides or amino acids, polyvinylidene difluoride (PVDF) membranes are the most ideal item for tracing down these transferred small molecular weight materials after electroblotting. The UltraScence PVDF/Filter Paper Sandwiches, 0.2 μm, have excellent binding properties for western blotting, dot-blot assays, and other protein or nucleic acid methods such as protein sequencing.

Specifications

Dimensions/Size: 7.3 cm x 8.3 cm

Material: PVDF membrane

Wettability: Hydrophobic

Thickness: 140~150μm

Pore Size: 0.2 μm

BSA Protein Binding Capacity: ~300 μg/cm²

Table 1. The protein binding capacity of the PVDF membrane.

The PVDF membranes were incubated in 0.5 mg/ml protein solution at room temperature for 24 hrs. Absorbance values were detected at 280 nm by the BCA assay, and the protein binding capacity can be calculated.

* PALL is a registered trademark of PALL Corporation. The trademark holder is not affiliated with Bio-HeliX Co., Ltd. and does not recognize this product.

Figure 1. The image of the PVDF membrane after wet transfer and semi-dry transfer of Tris-Glycine SDS-PAGE.