Nitrocellulose/Filter Paper Sandwiches, 0.2 μm, as high-quality membranes ideal for blotting proteins and nucleic acids, are ideal for the transfer of low molecular weight proteins (less than 20 kDa) and nucleic acids (less than 300 bp), exhibiting high sensitivity and low background for immunoblotting.

Specifications

Dimensions/Size: 7.3 cm x 8.3 cm

Material: Nitrocellulose membrane

Wettability: Hydrophilic

Thickness: 110-120 μm

Pore Size: 0.2 μm

Protein Binding Capacity:  ~200 μg/cm^{2 *}

* Incubation method with Glucose-6-phosphate dehydrogenase

Table 1. The protein binding capacity of the NC membrane.

The NC membranes were incubated in 0.5 mg/ml protein solution at room temperature for 24 hrs. Absorbance values were detected at 280 nm by the BCA assay, and the protein binding capacity can be calculated.

* PALL is a registered trademark of PALL Corporation. The trademark holder is not affiliated with Bio-HeliX Co., Ltd. and does not recognize this product.