
BIO-HELIX - CCH321-P10L
UltraScence Pico Plus Western Substrate Powder
Size: 5L x 2 / Set
The UltraScence Pico Plus Western Substrate Powder, as a luminol-based enhanced chemiluminescent substrate, is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugated secondary antibodies. The low picogram to low femtogram detection of antigen is enabled by UltraScence Pico Plus-Pico Ultra/Femto Western Substrate’s excellent sensitivity and long signal duration. Further, its long chemiluminescent signal duration makes both digital and film-based imaging possible without any loss of the signal. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.
Product Name: UltraScence Pico Plus Western Substrate Powder
Product Item No.: CCH321-P10L
Size: 5L x 2 / Set
Substrate Type: HRP (Horseradish Peroxidase) Substrate
Contents/Storage: Upon receipt make the ECL powder form into liquid form. It could be stable for up to 2 years at 25°C.
Patent No.: US10,711,185
- Ready-to-ship and significantly longer shelf life in the powder form!
- Significant reductions in transportation and storage costs, resulting in less carbon footprint!
- Empower custom production anywhere in the world!
- Significantly more sensitive than leading manufacturers on the market.
**US Patent No.: US10,711,185
Q: What’s the shelf life of UltraScence Pico Plus/Pico Ultra/Femto Western Substrate in liquid form?
A: 2 years at Room Temperature (25°C).
Q: What’s the shelf life of UltraScence Pico Plus/Pico Ultra/Femto Western Substrate in powder form?
A: Before you dissolve the powder ECL into liquid form, the shelf life could be more than 2 years in appropriate storage conditions. After you dissolve the powder ECL into liquid form, the shelf life of the liquid ECL: Store at RT (25°C) for 2 years.
Q: From Bio-Helix’s UltraScence ECL substrates portfolio, which level of ECL fits best my Western blot applications?
A: UltraScence ECL substrates series is compatible with the use from low picogram to low-femtogram level detections. Please kindly refer to the ECL selection guide of UltraScence Western substrate as the below table:
(a) CCH321-B100ML, UltraScence Pico Plus Western Substrate
(b) CCH345-B100ML, UltraScence Pico Ultra Western Substrate
(c) CCH365-B100ML, UltraScence Femto ECL Western Substrate
(d) CCH375-B100ML, UltraScence Femto Plus ECL Western Substrate
Q: What’s the volume and weight for the powder ECL substrates, CCH321-P10L?
A: Our powder ECL substrates, CCH321-P10L, (~200mg per package) could be dissolved to be 10L (=5L+5L) Western Substrate solutions.
Q: Do you have the comparison data of the ECL substrates between the liquid form and powder form? Does it perform in the same way?
A: There is an equal performance for both, liquid and powder forms. Please refer to the below table:
》Powder dissolution
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Prepare two 5L bottles. One amber bottle is for luminol solution, and other normal bottle is for peroxide solution. (Make sure the bottles are acid and alkali resistant.)
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Unpack the bag and pour all the powder in bottle.
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Rinse residual powder in bag with deionized water.
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Add deionized water to 5L.
》Reaction Setup
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Keep the membrane moist in the wash buffer while preparing the substrate mixture. Please ensure the membrane does not dry out during the subsequent steps.
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Mix Luminol solution and Peroxide Solution in a 1:1 ratio, and thoroughly agitate the chemiluminescent substrate solution well for preparing the 0.1 ml of solution / cm2 of membrane.
- For a mini-sized membrane (7 x 8.5 cm), 4 ml of solution is sufficient.
- For a midi-sized membrane (8.5 x 13.5 cm), 10 ml of solution is sufficient.
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Place the membrane with the protein side up on a clear and level surface or in a clean container.
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Remove the membrane from the chemiluminescent substrate solution and drain off excessive solution.
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Place the membrane in a plastic sheet protector or in plastic wrap to prevent the membrane from drying.
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Image the membrane with a digital imager or by exposing to the X-ray film.
Name | Download |
---|---|
PROTOCOL | CCH321_345_365_375-P10L_Protocol_v4_20220330.pdf |
Safety Data Sheet|SDS - Solution A | 2022_CCH321-P10L_CCH345-P10L_CCH365-P10L_CCH375-P10L_Solution_A_SDS.pdf |
Safety Data Sheet|SDS - Solution B | 2022_CCH321-P10L_CCH345-P10L_CCH365-P10L_CCH375-P10L_Solution_B_SDS.pdf |
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