The UltraScence Pico Plus Western Substrate Powder, as a luminol-based enhanced chemiluminescent substrate, is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugated secondary antibodies. The low picogram to low femtogram detection of antigen is enabled by UltraScence Pico Plus-Pico Ultra/Femto Western Substrate’s excellent sensitivity and long signal duration. Further, its long chemiluminescent signal duration makes both digital and film-based imaging possible without any loss of the signal. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.

Product Name: UltraScence Pico Plus Western Substrate Powder
Product Item No.: CCH321-P10L
Size: 5L x 2 / Set
Substrate Type: HRP (Horseradish Peroxidase) Substrate
Contents/Storage: Upon receipt make the ECL powder form into liquid form. It could be stable for up to 2 years at 25°C.

Patent No.: US10,711,185

-　Ready-to-ship and significantly longer shelf life in the powder form!

-　Significant reductions in transportation and storage costs, resulting in less carbon footprint!

-　Empower custom production anywhere in the world!

-　Significantly more sensitive than leading manufacturers on the market.

**US Patent No.: US10,711,185

Q: What’s the shelf life of UltraScence Pico Plus/Pico Ultra/Femto Western Substrate in liquid form?
A: 2 years at Room Temperature (25°C).

Q: What’s the shelf life of UltraScence Pico Plus/Pico Ultra/Femto Western Substrate in powder form?
A: Before you dissolve the powder ECL into liquid form, the shelf life could be more than 2 years in appropriate storage conditions. After you dissolve the powder ECL into liquid form, the shelf life of the liquid ECL: Store at RT (25°C) for 2 years.

Q: From Bio-Helix’s UltraScence ECL substrates portfolio, which level of ECL fits best my Western blot applications?
A: UltraScence ECL substrates series is compatible with the use from low picogram to low-femtogram level detections. Please kindly refer to the ECL selection guide of UltraScence Western substrate as the below table:

(a) CCH321-B100ML, UltraScence Pico Plus Western Substrate
(b) CCH345-B100ML, UltraScence Pico Ultra Western Substrate
(c) CCH365-B100ML, UltraScence Femto ECL Western Substrate

(d) CCH375-B100ML, UltraScence Femto Plus ECL Western Substrate

Q: What’s the volume and weight for the powder ECL substrates, CCH321-P10L?
A: Our powder ECL substrates, CCH321-P10L, (~200mg per package) could be dissolved to be 10L (=5L+5L) Western Substrate solutions.

Q: Do you have the comparison data of the ECL substrates between the liquid form and powder form? Does it perform in the same way?
A: There is an equal performance for both, liquid and powder forms. Please refer to the below table:

》Powder dissolution

1. Prepare two 5L bottles. One amber bottle is for luminol solution, and other normal bottle is for peroxide solution. (Make sure the bottles are acid and alkali resistant.)

2. Unpack the bag and pour all the powder in bottle.

3. Rinse residual powder in bag with deionized water.

4. Add deionized water to 5L.

》Reaction Setup

1. Keep the membrane moist in the wash buffer while preparing the substrate mixture. Please ensure the membrane does not dry out during the subsequent steps.

2. Mix Luminol solution and Peroxide Solution in a 1:1 ratio, and thoroughly agitate the chemiluminescent substrate solution well for preparing the 0.1 ml of solution / cm2 of membrane.

- For a mini-sized membrane (7 x 8.5 cm), 4 ml of solution is sufficient.
- For a midi-sized membrane (8.5 x 13.5 cm), 10 ml of solution is sufficient.

1. Place the membrane with the protein side up on a clear and level surface or in a clean container.

2. Remove the membrane from the chemiluminescent substrate solution and drain off excessive solution.

3. Place the membrane in a plastic sheet protector or in plastic wrap to prevent the membrane from drying.

4. Image the membrane with a digital imager or by exposing to the X-ray film.