As a recombinant human placental protein expressed in Escherichia coli, the RIBOAssure RNase Inhibitor inhibits ribonuclease (RNase) activity exhibited by eukaryotic enzymes such as RNase A, RNase B, and RNase C by non-covalent binding. It is intended for use in applications where the presence of RNases is undesirable for obtaining optimal RNA quality and experiment results, for example, RNA isolation, cDNA synthesis, RT-PCR, in vitro transcription and translation, or RNase-free monoclonal antibody preparation. RIBOAssure RNase Inhibitor lacks activity towards RNase 1, RNase T1, RNase T2, S1 nuclease, and RNase H. It is highly recommended to be used with our high-performance RScript Reverse Transcriptase, qPCR MasterMixes, Taq DNA polymerase, and/or 2X PCR SuperMix for subsequent experimental steps and applications.

Figure 1. Improved RNA stability in cDNA synthesis :
The interference of RNase under the experimental conditions will reduce the cDNA quantity produced through RNA conversion, resulting in a decrease in the subsequent RT-qPCR’s Ct value. After adding 4 units of RNase Inhibitor per reaction, the interference of RNase could be minimized.

Figure 2. RNase Inhibitor Temperature Resistance

The temperature resistance of RNase inhibitors was tested by incubating RIBOAssure RNase Inhibitor at various temperatures ranging from 25°C to 60°C for 15 minutes. 40 units of each inhibitor was subsequently used in reactions containing 2 µg of Mouse Liver Total RNA and 0.5 ng of RNase A. Positive and negative controls (first and second lanes, respectively) have no inhibitor added. Positive control contains RNA only. Negative control contains both RNA and RNase A.