The NEXT Fluor 11 Fluorescent Prestained Protein Ladder is a combination of 11 pre-stained proteins with molecular weights from 11 kDa to 300 kDa. The 11 recombinant proteins are stained with blue, red, and green dyes and also provide for fluorescent visualization, with 3 red bands at 27kDa, 80 kDa, and 300 kDa as reference bands. For fluorescent visualization, 10 stained proteins can be detected using the 440 nm – 460 nm excitation wavelength, and emission wavelengths at 700 nm – 740 nm. Primarily, the NEXT Fluor 11 Fluorescent Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.

Guide for Molecular Weight Estimation (kDa)

Refer to the NEXT Fluor 11 Fluorescent Prestained Protein Ladder patterns in various electrophoresis conditions:

Storage Buffer :

0.2~0.4 mg/ml of each band of protein is stored in a mixture of solution that contains: 20 mM Trisphosphate at pH 7.5, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.

Quality Control :
5 μl of NEXT Fluor 11 Fluorescent Prestained Protein Ladder (PMF11) resolves 11 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.

Storage :
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
Stable for up to 24 months at -20°C.

• Vertical Electrophoresis system
• Power supplies
• Vortex or equivalent
• Microcentrifuge

Figure 1. Image of PMF11 Fluorescent Prestained Protein Ladder for 4-20% TG gel, PVDF membrane, and fluorescent.

Each lane was loaded with 5 ul PMF11 Fluorescent Prestained Protein Ladder. For fluorescent visualization, 10 stained proteins can be detected using the 440 nm – 460 nm excitation wavelength, and emission wavelengths at 700 nm – 740 nm.

Lane 1: Thermo Scientific #26616 PageRuler Prestained Protein Ladder

Lane 2: Bio rad #1610373 Precision Plus Protein™ All Blue Prestained Protein Standards

Lane 3: NEXT Fluor 11 stored at 37 °C for 18 days ≈ -20°C for 912 days (2.5 years)

Lane 4: NEXT Fluor 11 stored at -20 °C for 18 days

Lane 5: NEXT Fluor 11 stored at 25 °C for 40 days ≈ -20°C for 912 days (2.5 years)